Immunochemical Characterization of Temperature - Regulated Production 1 of Enterocin L 50 ( EntL 50 A and EntL 50 B ) , Enterocin P , 2 and Enterocin Q by Enterococcus faecium L 50
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چکیده
Immunochemical Characterization of Temperature-Regulated Production 1 of Enterocin L50 (EntL50A and EntL50B), Enterocin P, 2 and Enterocin Q by Enterococcus faecium L50 3 4 5 Raquel Criado, Jorge Gutiérrez, María Martín, Carmen Herranz, 6 Pablo E. Hernández, and Luis M. Cintas 7 8 9 10 Departamento de Nutrición, Bromatología y Tecnología de los Alimentos, Facultad de 11 Veterinaria, Universidad Complutense de Madrid, 28040-Madrid, Spain. 12 13 14 Running title: Multiple bacteriocin production by E. faecium L50 15 16 17 18 19 * Corresponding author. Mailing address: Departamento de Nutrición, Bromatología y Tecnología 20 de los Alimentos, Avda. Puerta de Hierro s/n, Facultad de Veterinaria, Universidad Complutense 21 de Madrid, 28040-Madrid, Spain. Phone: +34-913943751. Fax: +34-913943743. E-mail: 22 [email protected] 23 AC CE PT ED Copyright © 2006, American Society for Microbiology and/or the Listed Authors/Institutions. All Rights Reserved. Appl. Environ. Microbiol. doi:10.1128/AEM.00983-06 AEM Accepts, published online ahead of print on 20 October 2006
منابع مشابه
Immunochemical characterization of temperature-regulated production of enterocin L50 (EntL50A and EntL50B), enterocin P, and enterocin Q by Enterococcus faecium L50.
Polyclonal antibodies with specificity for enterocin L50A (EntL50A), enterocin L50B (EntL50B), and enterocin Q (EntQ) produced by Enterococcus faecium L50 have been generated by immunization of rabbits with chemically synthesized peptides derived from the C terminus of EntL50A (LR1) and EntL50B (LR2) and from the complete enterocin Q (EntQ) conjugated to the carrier protein keyhole limpet hemoc...
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The locations of the genetic determinants for enterocin L50 (EntL50A and EntL50B), enterocin Q (EntQ), and enterocin P (EntP) in the multiple bacteriocin producer Enterococcus faecium strain L50 were determined. These bacteriocin genes occur at different locations; entL50AB (encoding EntL50A and EntL50B) are on the 50-kb plasmid pCIZ1, entqA (encoding EntQ) is on the 7.4-kb plasmid pCIZ2, and e...
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Enterococcus faecium L50 grown at 16 to 32 degrees C produces enterocin L50 (EntL50), consisting of EntL50A and EntL50B, two unmodified non-pediocin-like peptides synthesized without an N-terminal leader sequence or signal peptide. However, the bacteriocin activity found in the cell-free culture supernatants following growth at higher temperatures (37 to 47 degrees C) is not due to EntL50. A pu...
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